The Pine Bark Adelgid, Pineus strobi, Contains Two Novel Bacteriocyte-Associated Gammaproteobacterial Symbionts

Elena R. Toenshoff, Gitta Szabó, Daniela Gruber, Matthias Horn
2013 Applied and Environmental Microbiology  
ABSTRACTBacterial endosymbionts of the pine bark adelgid,Pineus strobi(Insecta: Hemiptera: Adelgidae), were investigated using transmission electron microscopy, 16S and 23S rRNA-based phylogeny, and fluorescencein situhybridization. Two morphologically different symbionts affiliated with theGammaproteobacteriawere present in distinct bacteriocytes. One of them ("CandidatusAnnandia pinicola") is most closely related to an endosymbiont ofAdelges tsugae, suggesting that they originate from a
more » ... e already present in ancient adelgids before the hosts diversified into the two major clades,AdelgesandPineus. The otherP. strobisymbiont ("CandidatusHartigia pinicola") represents a novel symbiont lineage in members of the Adelgidae. Our findings lend further support for a complex evolutionary history of the association of adelgids with a phylogenetically diverse set of bacterial symbionts.
doi:10.1128/aem.03310-13 pmid:24271164 pmcid:PMC3911223 fatcat:rkhpbh4ghjfknkvpc6qyux5vpi