Tas七 e じ e 丑s release neu mj 撫∋rs 血 1 . ∈ sponse to a し a5tant tbr sendl the signo. ls m gustaiky n 巳 we fibers 、 nt TeportB 1旧 ve highlighted the role of . 4Tl ) as a key neumtratusmitter iom taste ce ] ls to gus匸abDry ne じ ve 6bers. Howev 隅 A ' IP release plope血 es ortas し e cell5 and the med 旧 ni5 s have Iloちbeen elucidatod . Hore we ed m detoct tastan 匸 . ε voked ATP reLease m s岬 e 幡 te eells with a 曲 n putentinls oE mouse 血皿 幽 pa曲 e . ' lhe a [tion pDtentials were recorded " 重 th the

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... ede baso 且 aterally attH : hed 亡 o a s 血 gle 仁aste cel ユ . Th 巳 electrude soluhDn wa 昌 collettea and apphed ror luciferaLse [ Ypc [ [cellS expreES eweA1 . , b 撫 r and um i ltlsto lumptOrs ana mrductinn components but a ) nventional s} pse5 have not bLen obs 巳rved in tllese c 曲 . ( } n 丗 e other hane Type HI eele PS 〕 ssess 跚 ユ apses but not 出 ese tasberelated lllniecLiles . Retentl } ;tas し e 副 s eXPressing makers for TSpe 皿 detS were shown tO be co ' expressed 、 、 dth putative gour tasterecel ] tOrs , sugges し 血g sりme 塾 ・ pe 皿 cenS inay be involved in soirr senMtion . HowevgT , little is 且 mowtl abOu し ph ) ' sio ] 09ical reSPOIIses of TSpe [ ce 臨 and 覧 脚 馴I eells . 【 n 恤 is s 加 略- wn 血 vestigated response propenies ef speCified taste ce ] ls by mean § oftrallsge = mice Which e 耳pTesE g een fiuovesceut protein〔 G止 1 [ ' ) in gUsrdunin . 1 〕 ositive 〔 T } 1 〕 e m and GAD67 ・ poSitive ( Tkpe IID UB . By uLsinLj a lor 〕 se pateh rmrding 亡 que , we tbtmd spantaneDus firillg of ac じ bn 【 x)匸en 匸眺 in GFP e耳plessing tasし e oen50f both Gu5U 加 chl . GFP and GAI 〕 67 . GFP mice . 翫 me Df Gusrdudn . GFP eElls mB 凶 nded tO saoCharin and o 由 er sweeteners , suggegting thnt a palt αf 「 lypε ∬ c曲 may be sweet 匸 aste r epbDr eeHs . 鼠)me of GAD67 − GFP ceUs showed sE児 c丗c responses ω so し n . taBte stimull su ¢ h as HCL mid d 由 c a 皿 su 継 血 g t a p 凪 of 野 pe 皿 ce 出 may be 鼬 財鵬 艶 recepbDr Us. 「 エ hese i℃ sultS suggest tlll ユ し 恥 し h し ype H and Type m eell5 mo . y be tas 匸 e roreptnr ealls tha亡are devDted亡 o 〔 皿 brenr . taste qusTities . Suplx ) r 囲 by JSPS C: rznts . 血 . Aid IS 〕77004, ISIDSO13 ( YN)and 1979L? 67 、 hl respem . ge tn va 「 in −s stirnulL VOnb ti1 血 e 。 DntrolS 丗 e 蹴 a 七bn Uf its 曲 9且 e μ ) 且 ar 且 ag 曲 m b〕migratetowartl merefavuruble eriviivirmemts 、 an ability knowni as cbemotaxis whicli 蕊 phcated in pathogeniciby ・ as well as survivo. 1in vari σ し Lg e11 伽 nlnents . The 『 伽 纏 騨 me i副 蹴 d 加 de 45 che [ − p 曲 晦 , wbch we e methyL . accep 血1gchom σ曲 p 蹠 勇 n . Hkep飜 hls呱皿 s ) . Hovifever , itiSlarge ! ytmbown what d ofs 血 蚰 tllese pu 膩 ive chem 3ceptQrs se . W6 ve し herebre mlched to explcre 丗 e 丘血