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A tissue culture protocol using leaf sheath as the explants has been established for callus induction and for plant regeneration of eastern gamagrass. An MS basal medium supplemented with 2,4-D, kinetin, and zeatin was used to induce friable calli and to form plantlets in vitro. Ethylmethane sulfonate applied at a concentration of 0.01% on calli appeared to be effective in inducingdoi:10.1508/cytologia.63.381 fatcat:lroj3zwct5arnafzjrieq4mmhm