A Note on the Susceptibility of Ferrets to the Virus of the Common Cold

W. C. Noble, D. H. Brainard
1935 Journal of Bacteriology  
Future progress in the prevention and treatment of the common cold depends largely on the discovery of susceptible animals to which colds can be transmitted for the purpose of studying the immunity reactions elicited. Dochez et al. (1930) have shown that chimpanzees are susceptible; but the size and high cost of these animals prohibit their use by most experimental workers. Smith, Andrewes, and Laidlaw (1933) found that ferrets could be infected with influenza virus, and report successful
more » ... rt successful transmission with the throat washings from 5 of 8 influenza patients. They were unable to infect their animals with the virus of the common cold, although it is probably related to that of influenza. As they made but one attempt, however, it seemed desirable to us to study further the susceptibility of ferrets to the cold virus. Nasopharyngeal washings were collected from persons with fresh colds, filtered immediately through Berkefeld "W" candles and the filtrates used to inoculate ferrets. As a rule, the filtrates of combinedwashingsfrom several persons were injected into each animal, 0.5 cc. subcutaneously and 0.5 cc. intranasally. As controls, other ferrets were given sterile broth. Temperatures were noted and the animals examined twice daily for signs of respiratory disease. Every animal was kept carefully isolated for two weeks before, and during the experimental period to rule out extraneous infections of the respiratory tract. At no time did~any of the stock animals exhibit symptoms of respiratory disease. Filtrates from 40 persons with colds were injected into 12 fer-407 on May 9, 2020 by guest
doi:10.1128/jb.29.4.407-409.1935 fatcat:n72fj3l3sbhbfnb4byhta6gmqu