This study investigated the bacterial diversity associated with microbial mats of polar deep-sea cold seeps. The mats were associated with high upward fluxes of sulfide produced by anaerobic oxidation of methane, and grew at temperatures close to the freezing point of seawater. They ranged from small patches of 0.2-5 m in diameter (gray mats) to extensive fields covering up to 850 m 2 of seafloor (white mats) and were formed by diverse sulfide-oxidizing bacteria differing in color and size.

more »

... all, both the dominant matforming thiotrophs as well as the associated bacterial communities inhabiting the mats differed in composition for each mat type as determined by microscopy, 16S rRNA gene sequencing and automated ribosomal intergenic spacer analysis. While the smaller gray mats were associated with a highly diverse composition of sulfide oxidizers, the larger white mats were composed of only 1-2 types of gliding Beggiatoa filaments. Molecular analyses showed that most of the dominant mat-forming sulfide oxidizers were phylogenetically different from, but still closely related to, thiotrophs known from warmer ocean realms. The psychrophilic nature of the polar mat-forming thiotrophs was tested by visual observation of active mats at in situ temperature compared to their warming to > 4 • C. The temperature range of mat habitats and the variation of sulfide and oxygen fluxes appear to be the main factors supporting the diversity of mat-forming thiotrophs in cold seeps at continental margins. Published by Copernicus Publications on behalf of the European Geosciences Union. Fluorescence in situ hybridization (FISH) experiments Sediment samples were fixed in 4 % formaldehyde/seawater, washed twice with 1 × phosphate-buffered saline (PBS; pH 7.2 to 7.4), and were stored at −20 • C in a 1 : 1 mixture of PBS and ethanol until further processing. Catalyzed reporter deposition FISH (CARD-FISH) was carried out with suitable Biogeosciences, 9, 2947-2960, 2012 www.biogeosciences.net/9/2947/2012/