Plate cell – SELEX v2 (protocols.io.8sehwbe) [dataset]

Claudia Troncone
2019 protocols.io  
1 1 AEGIS -Madrid iGEM 2019 Aptamers are short single-stranded oligonucleotide (DNA or RNA) molecules with the ability to bind to other molecules with high affinity and specificity. Nowadays, aptamers are used as biosensing molecules with broader applications, such as ribozymes (RNA enzyme molecules with catalytic activities); riboswitches (modulating translational activities) and ligands, recognizing specific target binding. Aptamers are folded, directed by their sequences, acquiring specific
more » ... acquiring specific 3D conformations. This 3D conformation property has been widely applied in order to recognize cell structures. Aptamers evolve from random oligonucleotide pools by a process called Syste ma ti c Evol uti on of L i ga nds by Expone nti a l Syste ma ti c Evol uti on of L i ga nds by Expone nti a l
doi:10.17504/protocols.io.8sehwbe fatcat:phjwe2uwyzfohf54rkveyd7jwy