Novel optical detection system for in vivo identification and localization of cervical intraepithelial neoplasia

Kevin T. Schomacker, Thomas M. Meese, Chunsheng Jiang, Charles C. Abele, Karen Dickson, Stephen T. Sum, Ross F. Flewelling
2006 Journal of Biomedical Optics  
A noncontact optical detection system is developed for the in vivo identification and localization of high-grade cervical intraepithelial neoplasia ͑CIN 2,3͒. Diagnostic scans of the entire human cervix are performed following acetic acid application employing three integrated optical measurements: laser-induced fluorescence spectroscopy, white light diffuse reflectance spectroscopy, and video imaging. Full cervical scans comprising 499 interrogation locations at 1-mm spatial resolution are
more » ... leted in 12 s. Diffuse reflectance and fluorescence spectra with signal-to-noise ratios of better than 100-to-1 are collected between 360 and 720 nm in increments of 1 nm, with an inherent spectral resolution of 8 nm. Glare reduction and optical vignetting are handled with a novel illumination scheme and subsequent spectral arbitration algorithms. The system is designed and found to be well below acceptable safe optical exposure levels. Typical reproducibility across multiple systems is approximately 5%, providing reliable and accurate detection of in vivo cervical neoplasia in normal clinical use.
doi:10.1117/1.2208987 pmid:16822059 fatcat:u3d3ufzcobgnngt7wxeu5qgpzy