The side-effect of Azadirachta indica (A. Juss) leaf extract on certain biochemical parameters of a freshwater fish, Labeo rohita was studied for a period of 25 days at sublethal concentration. The median lethal concentration (1.035 g/L) of A. indica for 24 h was calculated and 1/10 th of the toxicant (0.1035 g/L) was taken for sub-lethal study. During the exposure period the glycogen content in the liver and muscle of leaf extract treated fish increased up to the 15 th day and then declined,

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... ereas the protein level decreased in the liver and muscles throughout the study period. The alterations of these biochemical parameters can be effectively used as non-specific biomarkers against plant extract toxicity stress and also help safer usage of plant extracts in aquaculture farms.