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A cytotoxic aldehyde, 4-hydroxy-2E-nonenal (4-HNE), is formed by lipid peroxidation in cells. In order to investigate formation of 4-HNE and the related aldehyde, 4-hydroxy-2E-hexenal (4-HHE) in cooking oil, we developed a quantitative analytical method for the aldehydes using synthesized deuterium labeled aldehydes as the internal standards. The cooking oil including internal standards was clean-upped by a cartridge-type silica-gel column, and the hydroxyl groups of 4-HNE and 4-HHE weredoi:10.24514/00002892 fatcat:rbgthn22dzhirbkj33fhubfqii