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Few identification methods will rapidly or specifically detect all bacteria in the order Chlamydiales, familyChlamydiaceae. In this study, three PCR tests based on sequence data from over 48 chlamydial strains were developed for identification of these bacteria. Two tests exclusively recognized theChlamydiaceae: a multiplex test targeting theompA gene and the rRNA intergenic spacer and a TaqMan test targeting the 23S ribosomal DNA. The multiplex test was able to detect as few as 200doi:10.1128/jcm.37.3.575-580.1999 fatcat:sh7r2n7s6jfc3ohisyqsluapuy