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The promoters recognized by or7r, the primary sigma ofEscherichia coli, consist of two highly conserved hexamers located at -10 and -35 bp from the start point of transcription, separated by a preferred spacing of 17 bp. a, factors have two distinct DNA binding domains that recognize the two hexamer sequences. However, the component of RNA polymerase recognizing the length of the spacing between hexamers has not been determined. Using an equilibrium DNA binding competition assay, we demonstratedoi:10.1073/pnas.93.17.8858 pmid:8799117 pmcid:PMC38558 fatcat:vx53lpigkvbgfczlkastlio5su