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Comprehensive introduction to the processing and analysis of bulk RNA-seq data including basic information about Illumina-based short read sequencing, common file formats (FASTQ, SAM/BAM, BED, ...) and quality controls. Contains ready-to-use UNIX and R code; covers the most common application of bulk RNA-seq to identify genes that are differentially expressed when comparing two conditions.doi:10.5281/zenodo.4390117 fatcat:cijrio4upzas5ovxw6yezqtbka