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Separation and identification of selenoproteins in selenium-enriched yeast
셀레늄이 강화된 이스트에서 셀레늄 단백질의 분리 및 확인
2013
Analytical Science and Technology
셀레늄이 강화된 이스트에서 셀레늄 단백질의 분리 및 확인
Preserved Fulltext
Selenium-containing proteins were separated from selenium-enriched yeast (SEY) using Trizol ® reagent followed by anion exchange (AE) chromatography. This method is simpler and less time consuming than electrophoresis. Five selenium containing proteins were identified by on-line AE HPLC-ICP/MS (high performance liquid chromatography-inductively coupled plasma/mass spectrometry). Each protein was enzymatically hydrolyzed to seleno-amino acids and separated with RP (reverse phase) HPLC for the identification of selenoproteins.
doi:10.5806/ast.2013.26.6.357
fatcat:73wxtevohzeuxg4q2rfzkn5y3y