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To evaluate the fungal diversity in the Pirarungaua stream reopened after remaining channeled about 40 years in the Botanical Garden of São Paulo, Brazil, two experiments in the cold and dry season and two in the hot and rainy season were performed. Leaves of Tibouchina pulchra Cogn and mixed leaf litter were confined in nylon litter bags, submerged and collected monthly, together with free leaf litter samples. Some abiotic factors were measured in the water. The leaves were fragmented,doi:10.1590/2236-8906-58/2015 fatcat:dwwcznwiijcw7fj6aqqigylxqm