Biochemical control of fresh-water sponge development: effect on DNA, RNA and protein synthesis of an inhibitor secreted by the sponge

F Rozenfeld
1974 Journal of embryology and experimental morphology  
During their growth, fresh-water sponges release into the medium a dialysable inhibitor (gemmulostasin) which reversibly blocks development of the gemmules at an early phase, prior to the first mitoses. The incorporation of [ 3 H]labelled precursors into DNA, RNA and proteins has been measured throughout development, in the presence or absence of inhibitor. Autoradiographic controls of the nuclear incorporation of [ 3 H]thymidine have been made. Gemmulostasin erases the peak of [ 3 H]thymidine
more » ... of [ 3 H]thymidine incorporation that otherwise occurs just before the first mitoses. Its overall effect is to bring about, either directly or indirectly, a high peak in the incorporation of [ 3 H]uridine and [ 3 H]leucine at a time when the incorporation of these precursors is low in the controls. It is suggested that a causal relation exists between these phenomena.
pmid:4463206 fatcat:5rwuifmy2vhb3k7jn4r3m7r7ty