A Novel Improved RP-HPLC Method for the Estimation of Dothiepin Hydrochloride in Bulk and Pharmaceutical Dosage Form

Vijaya lakshmi. M, M. Srinavyatha, Lakshmi Prasanna. M, Buchi N. Nalluri
2017 IOSR Journal of Pharmacy and Biological Sciences  
A Simple and precise reverse phase high performance liquid chromatography(RP-HPLC)method was developed and validated for the estimation of Dothiepin hydrochloride in bulk and pharmaceutical dosage form. The optimization is carried out on an Inertsil ODS 3 (250×4.6mm, 5µ particle size) long column with a mobile phase of Formic acid(0.02%): Acetonitrile in the ratio of 72:28v/v at a flow rate of 1ml/min with UV detection at 230 nm using LC solution software. The Linearity of Dothiepin
more » ... thiepin hydrochloride was observed in the concentration range of 2-10µg/ml (R 2 =0.999). The proposed method has shown consistent recovery of Dothiepin 99.41-100.85% with the labeled amount in the pharmaceutical formulation. The proposed method was successfully applied for the quantification of active drug in tablet dosage form.
doi:10.9790/3008-1203012126 fatcat:yjkqlzit25fcdg5tyuoqpt36lm