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A Simple and precise reverse phase high performance liquid chromatography(RP-HPLC)method was developed and validated for the estimation of Dothiepin hydrochloride in bulk and pharmaceutical dosage form. The optimization is carried out on an Inertsil ODS 3 (250×4.6mm, 5µ particle size) long column with a mobile phase of Formic acid(0.02%): Acetonitrile in the ratio of 72:28v/v at a flow rate of 1ml/min with UV detection at 230 nm using LC solution software. The Linearity of Dothiepindoi:10.9790/3008-1203012126 fatcat:yjkqlzit25fcdg5tyuoqpt36lm