Biosynthesized nanoparticles have a huge perspective. It is an environment-friendly, cost-efficient, reproducible, and energy-efficient method compared to physical or chemical synthesis. In the present study, biosynthesis of copper oxide nanoparticles (CuONP) was done using Purpureocillium lilacinum. Characterization of synthesized CuONP was done by using UV–vis spectroscopy, TEM, and XRD analysis. UV–vis gave characteristic SPR peak for CuONP at 360 nm. TEM image reveals that the morphology of

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... biosynthesized CuONP was spherical, and their size range between 4.03nm to 8.83nm. The XRD analysis confirmed the crystalline nature of CuO with a size range of 6-26.6nm. Further comparative study of photocatalytic degradation of navy blue and safranin using CuONP was done. CuO-NPs exhibited potential catalytic activity in navy blue (57.5 %) and safranin (63 %), respectively.