Recebido em 30/1/08; aceito em 20/6/08; publicado na web em 31/10/08 EXTRACTION AND ACTIVITY OF PEROXIDASE IN ARAZÁ (Eugenia stipitata Mc Vaugh) PULP FRUIT. A technique for both extraction and activity measurement of peroxidase extracted from arazá (Eugenia stipitata Mc Vaugh) is described. Peroxidase from arazá pulp fruit was extracted using a combination of protein precipitation with acetone and extraction with 50 mM sodium buffer phosphate (pH 6.0). Optimum activity using guaiacol as H-donor

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... was obtained at pH from 5.0 to 6.5, temperature from 60 to 75 °C, H 2 O 2 between 10 to 15 mM and guaiacol from 80 to 160 mM. Thermal inactivation showed a first-order inactivation kinetic. Reactivation was observed when extracts were heated at 80 °C and afterwards incubated at 25 °C.