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While the vast majority of spliceosomal introns are removed by the so-called U2 (major) spliceosome, diverse eukaryotes also contain a mysterious second form, the U12 (minor) form, and associated introns. In all characterized species, U12-type introns are distinguished by several features, including being rare in the genome, containing extended evolutionary-conserved splicing sites, being generally ancient as judged by conservation between distant species, and being inefficiently spliced. Here,doi:10.1101/2020.10.12.336362 fatcat:2da245xmgjdulk2kz6hxn7525u