This study was intended to determine rubella virus seroprevalence in pregnant women. The samples included 240 women who attended during the duration from October 2018 to May 2020 Al-Batool Gynecology Hospital in Baqubah District. Women's age ranged from 16-45 years; their age mean was 26.38 years. Data including age, number of previous abortions. Sera were collected from each woman, and the levels of rubella IgG and IgM were detected by use Electrochemiluminescence (ECLIA), enzyme-linked

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... orbent assay (ELISA) and On Site Rubella IgG/IgM Rapid (OnSite) tests. Also, RT-PCR test are used to detect rubella viral RNA. The results revealed that anti-rubella IgG antibodies for the 240 pregnant women were ranges from 84.58 % to 89.58 % depending on the method tested used. While the anti-rubella IgM antibodies for the 240 pregnant women were ranges from 5 % to 5.41 % depending on the method tested used. Only 8 from 240 specimens of pregnant women are rubella viral RNA positive with (3.33 %) percentage by RT-PCR test. In relation to the history of abortion, the seropositivity of rubella antibodies were (61.42 %) of pregnant women had history of abortion with significant relation P< 0.05, however the abortion frequency among Pregnant patients have the past of one or more abortions with the nonsignificant P>0.05 relation.