Data from: Spectroscopic approach to correction and visualisation of bright-field light transmission microscopy biological data [article]

Ganna Platonova, Dalibor Štys, Pavel Souček, Kirill Lonhus, Jan Valenta, Renata Rychtáriková
2021 Zenodo  
The most realistic information about the transparent sample such as a live cell can be obtained only using bright-field light microscopy. At high-intensity pulsing LED illumination, we captured a primary 12-bit-per-channel (bpc) response from an observed sample using a bright-field wide-field microscope equipped with a high-resolution (4872x3248) image sensor. In order to suppress data distortions originating from the light interactions with undesirable elements in the optical path, poor sensor
more » ... reproduction (geometrical defects of the camera sensor and some peculiarities of sensor sensitivity), this uncompressed 12-bpc data underwent a kind of correction after simultaneous calibration of all the parts of the experimental arrangement. Moreover, the final intensities of the corrected images are proportional to the photon fluxes detected by a camera sensor. It can be visualized in 8-bpc intensity depth after the Least Information Loss compression [Lect. Notes Bioinform. 9656, 527 (2016)].
doi:10.5281/zenodo.4843453 fatcat:ln6bte7fujc3vkedp7tasgubge