Myotendinous stiffness is related to the collagen content of the muscle and tendon, and can be estimated during running by changes in vertical stiffness (kvert) and the resulting modifications of the spatiotemporal parameters (on-off ground asymmetry and landing-takeoff asymmetry). Supplementation with amino acids found in collagen, such as proline, glycine, and hydroxyl proline, combined with ascorbic acid, improve collagen synthesis and potentially result in improved mechanical strength and

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... iffness. Objectives: To determine if hydrolyzed collagen (HC) supplementation increases kvert and improves the spatiotemporal parameters during running in recreational triathletes. Methods: Nine active males (weight; 68.4 ± 5.7 kg, height; 171.8 ± 5.4 cm, age; 32.5 ± 4.1 years; Vo2max; 53.15 ± 2.19 mL/kg/min) were randomly distributed into a collagen group (CollG, n = 5) and a control group (CG, n = 4). Participants were supplemented for 4 weeks with 15g HC (CollG) or 15g placebo (CG; maltodextrin), 3 times per week. One hour after supplementation, the participants of both groups were asked to perform four repetitions of short sprints to further stimulate collagen synthesis. The ground reaction forces were recorded during running at 4.44 m s-1, 5.55 m.s-1, and 6.66 m.s-1 for assessment of kvert and the spatiotemporal step parameters. Results: Both groups increased kvert with speed (4.44 - 6.66 m s-1) from 24.8 ± 2.7 to 53.7 ± 16.5 N/m and from 25.1 to 49.8 N/m in the CollG and CG, respectively (P < 0.0001); however, there were no differences between groups before and after the supplementation period. As a consequence, the spatiotemporal parameters of running were also similar between groups. Conclusions: Four weeks of HC supplementation does not improve the bouncing mechanism of running in recreational triathletes.