Toxicity of Aqueous Fullerene nC60to Activated Sludge: Nitrification Inhibition and Microtox Test

Yongkui Yang, Norihide Nakada, Ryoji Nakajima, Chao Wang, Hiroaki Tanaka
2012 Journal of Nanomaterials  
The increasing production and use of fullerene nanomaterials raised their exposure potential to the activated sludge during biological wastewater treatment process. In this study, the toxicity of aqueous nanoscaled C60(nC60) to activated sludge was investigated using nitrification inhibition and Microtox test. The test solutions of nC60were prepared using two methods: long stirring (Stir/nC60) and toluene exchange (Tol/nC60). The nC60aggregation in test medium was also evaluated for toxicity
more » ... essment. The results showed that the nC60aggregation behaved differently in two test mediums during the incubation periods. The nC60toxicity was greatly influenced by the preparation method. Stir/nC60presented no significant toxicity to both the nitrification sludge and bioluminescent bacteria at the maximum concentration studied. In contrast, the EC20of Tol/nC60was obtained to be 4.89 mg/L (3 h) for the nitrification inhibition and 3.44 mg/L (30 min) for Microtox test, respectively.
doi:10.1155/2012/512956 fatcat:mkaod3y7nvdtrasrqzwfapdigi