Construction of pETNF-P16 plasmid and its expression properties in EC9706 cell line induced by X-ray irradiation

Cong-Mei Wu
2004 World Journal of Gastroenterology  
AIM: Recombined plasmid pETNF-P16 was constructed to investigate its expression properties in esophageal squamous carcinoma cell line EC9706 induced by X-ray irradiation and the feasibility of gene-radiotherapy for esophageal carcinoma. METHODS: Recombined plasmid pETNF-P16 was constructed and transfected into EC9706 cells with lipofectamine. ELISA, Western blot, and immunocytochemistry were performed to determine the expression properties of pETNF-P16 in EC9706 after transfection induced by
more » ... ay irradiation. RESULTS: Eukaryotic expression vector pETNF-P16 was successfully constructed and transfected into EC9706 cells. TNFα expressions were significantly increased in the transfected cells after different doses of X-ray irradiation than in those after 0Gy irradiation (1 192.330-2 026.518 pg/mL, P<0.05-0.01), and the TNFα expressions and P16 were significantly higher 6-48 h after 2 Gy X-ray irradiation (358.963-585.571 pg/mL, P<0.05-0.001). No P16 expression was detected in normal EC9706 cells. However, there was strong expression in the transfected and irradiation groups. CONCLUSION: X-ray irradiation induction could significantly enhance TNFα and P16 expression in EC9706 cells transfected with pETNF-P16 plasmid. These results may provide important experimental data and therapeutic potential for gene-radiotherapy of esophageal carcinoma. Wu CM, Huang TH, Xie QD, Wu DS, Xu XH. Construction of pETNF-P16 plasmid and its expression properties in EC9706 cell line induced by X-ray irradiation. World J Gastroenterol
doi:10.3748/wjg.v10.i20.2927 pmid:15378766 pmcid:PMC4576245 fatcat:sodnknj4k5h2fble7n7kdtyrt4