Effects of oxidative stress on liver, brain and spinal cord of rats using L-NAME and treated with hydroxyurea. A model of sickle cell complication

Abilio Torres dos Santos Neto, Iandara Schettert Silva, Maria Lucia Ivo, Camila Tozaki Rodrigues, Eduardo Benedetti Parisotto, Rondon Tosta Ramalho, Geanlucas Mendes Monteiro
2020 Acta Cirurgica Brasileira  
To analyze the serum levels of nitric oxide and correlate them with the levels of thiobarbituric acid reactive substances (TBARS) in liver, brain and spinal cord of animals using L-NAME and treated with hydroxyurea. Eighteen male albino Wistar rats were divided into three groups. NG-nitro-L-arginine methyl ester (L-NAME) was intraperitoneally administered to induce oxidative stress. TBARS and plasma nitric oxide levels were analyzed in all groups. Histopathology of the liver and vascular tissue
more » ... and vascular tissue was performed. Statistically significant differences were seen in liver, brain and spinal cord TBARS levels. Following the use of L-NAME, hepatic tissue increased the number of Kupffer cells as oxidative stress and inflammatory response increased. The use of L-NAME caused an increase in lipid peroxidation products and, consequently, in oxidative stress in animals. Hydroxyurea doses of 35 mg / kg / day reduced TBARS values in liver, brain and spinal cord.
doi:10.1590/s0102-865020200030000001 pmid:32401830 fatcat:4hxvb755wfhsjj5ypzsbmq4bea