Cellular Signaling Mechanisms Underlying Pharmacological Action of Bak Foong Pills on Gastrointestinal Secretion

J. X. Zhu, Y. M. Chan, L. L. Tsang, L. N. Chan, Q. Zhou, C. X. Zhou, H. C. Chan
2002 The Japanese Journal of Physiology  
Bak Foong Pills (BFP, also known as Bai Feng Wan) is an over-the-counter traditional Chinese medicine (China registration No. Z980035) that has long been used for treating gynecological disorders and improving of overall body functions, including gastrointestinal (GI) function [1] . However, the cellular signaling mechanism underlying BFP action, especially on the GI tract, has not been elucidated. Secretagogue-induced Cl Ϫ secretion by the GI tract is important because Cl Ϫ secretion provides
more » ... secretion provides an essential driving force for the lubrication of intestinal contents during regular bowl movements or the flushing of microbial organisms or artificial irritants in host defense responses [2, 3] . Epithelial Cl Ϫ channels, especially the cAMP-activated Cl Ϫ channels, play an important role in regulating and maintaining the normal physiological functions of the GI tract. The abnormal regulation of Cl Ϫ channels, such as by cholera toxin, Abstract: Bak Foong Pills (BFP, also known as Bai Feng Wan) is an over-the-counter traditional Chinese medicine that has long been used for treating gynecological disorders and improving overall body functions, including gastrointestinal (GI) function. However, the cellular signaling mechanism underlying BFP action, especially on the GI tract, has not been elucidated. In the present study, the human colonic epithelia cell line T 84 was used as a model to investigate the effect of BFP ethanol extract on ion transport in conjunction with the short-circuit current (I SC ) technique. The results showed that the apical addition of BFP extract produced a concentration-dependent (10-1,000 g/ml, EC 50 ϭ120 g/ml) increase in I SC . The maximal response was observed at 500 g/ml with an increase in I SC of 24.4Ϯ2.3 A/cm 2 and apical conductance. The BFP-induced I SC was not observed when extracellular Cl Ϫ was replaced or when treated with Bumetanide (100 M), an inhibitor of the Na ϩ -K ϩ -
doi:10.2170/jjphysiol.52.129 pmid:12047811 fatcat:gvtm4qjlpnfnretmfgt6uqwqoe