During the investigation of accidental cases, blood samples obtained from accidental victim are submitted to forensic science laboratory for toxicological analysis. All through, ethanol analysis is performed on almost all cases of blood samples. The main problem is determining alcohol concentration in these cases is changes in Blood Alcohol Concentration (BAC) that can occur due to neoformation of ethanol. Blood samples stored under insufficient conditions or for longer times and body fluids

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... e undergone petrifaction often contains certain amount of volatile compounds. In the light of possibility that, neoformation of ethanol in improperly stored blood might give rise to an incorrect estimation of BAC. With this in mind, to study the effect of preservative and temperature on the formation and concentration of ethanol in blood samples, we estimated the ethanol neogenesis in stored and normal blood in different time period (0, 7, 14, 21, 28, 35 and 42 days). Head Space-Gas Chromatography instrument used to measure the level of ethanol in blood sample. We found that neoformation of ethanol was observed in normal blood sample. There was no any neoformation observed in blood sample with preservative and blood stored at 4°C. Our study is supporting the studies which suggest that storage condition will affect the neoformation of ethanol in blood samples.