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Mass transport has generally been recognized as a major limiting factor in the sensitivity and performance of miniaturized sensor platforms. To overcome this limitation, a new approach, termed molecular dam, has been developed to enhance mass transport for protein enrichment in nanofluidic channels by nanoscale electrodeless dielectrophoresis under physiological buffer conditions. We demonstrated protein enrichment factor to be greater than 10 5 -fold in 20 seconds, which is orders of magnitudedoi:10.21236/ada570888 fatcat:h5k6kjz7zjcpzdwkiml2vueuea