Determination of Biological Nitrogen Fixation Induced N2O Emission from Arable Soil by Using a Closed Chamber Technique
Applied and Environmental Soil Science
Intensive use of mineral N fertilizers and organic amendments has resulted in higher N2O emissions. A growing worldwide concern for these problems has motivated researchers, environmentalists, and policy makers to find alternatives to overcome such losses. Biological nitrogen fixation is one of many natural biological approaches to minimize the use of fertilizers and to possibly reduce N2O emissions. A greenhouse study was performed by growing inoculated and noninoculated soybean seeds (Glycine
... bean seeds (Glycine max (L.) Merr.) in PVC columns. The objective was to measure the contribution ofBradyrhizobium Japonicumand mineral-N fertilizer to promoting N2O emission. A closed chamber technique was used for gas sampling. N2O measurements were carried out shortly after nodulation.Bradyrhizobium Jopanicuminduced N2O cumulative (121.8 μg kg−1) fluxes of inoculated seeds was significantly (α= 0.05) higher than those of mineral N fertilized treatment (NIS) and the control (bare soil). Total nitrogen content of the roots and seeds was not affected by inoculation. Total carbon ( 42.1 ± 0.1%), total nitrogen (3.1 ± 0.1%), and crude protein (19.9 ± 0.7%) contents of leaves of the inoculated seeds were significantly higher than those of noninoculated seed treatments. N2O fluxes significantly increased with high dissolved organic carbon content (70.77 ± 3.99 mg L−1) at R3 and at R8 stages whenNO3-(39.60 ± 0.94 mg L−1) concentrations were high.