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Purpose: To synthesize thiosemicarbazide and determine its antimicrobial properties. Methods: Pyridine-based thiosemicarbazide was synthesized, characterized and evaluated for antimicrobial activity. The structure of the synthesized compound was established by spectral analysis, namely, Fourier transform infrared spectroscopy (FT-IR), proton nuclear magnetic resonance spectroscopy (1H NMR), carbon 13 magnetic resonance spectroscopy (13C NMR), liquid chromatography-mass spectroscopy (LC-MS),doi:10.4314/tjpr.v15i8.22 fatcat:vyoymu3aezefbcrlwdueeelexa