As many as 39 plant samples representing nine different botanical families, showing symptoms like virus diseases, were collected from different locations of Bangladesh in 1986-X7. The samples were preserved at 4°C after drying by lyophilization or over calcium chloride. Double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) and dot-immunobinding assay (DIBA) were applied for serological detection of viruses by using 11 different antiivirus-sera. Many of the samples (28/39)

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... positive reaction with either of the antisera used. In all eight different viruses, such as tobacco mosaic virus-ordinary strain (TMVOM), turnip mosaic virus (TuMV), broad bean wilt virus (BBWV), Shallot latent virus (SLV), leek yellow stripe virus (LYSV), tobacco rattle virus (TRV), papaya ringspot virus (PRSV) and mungbean yellow mosaic virus (MYMV) were serologically detected. Among these, mixed infection of SLV and LYSV were detected from garlic sample. Mixed infection of TuMV and BBWV were also detected from the sample of Chinese cabbage. TMVOM were found to be common in various plant species while PRSV, TuMV, TRV, MYMV and both SLV and LYSV were detected from each of papaya, Chinese cabbage, lettuce, okra and garlic samples, respectively. The results suggest that the dried samples stored for 2-3 years retained antigenicity of the viruses which could be detected by serological methods like DIBA and DASELISA. 121