Identification of serotonin-sensitive aryl acylamidase activity with cobra venom acetylcholinesterase

Ramanna V Rajesh, Aiylam S Balasubramanian, Rathanam Boopathy
2003 Indian Journal of Biochemistry & Biophysics  
Acetylcholinesterase purified from cobra (Naja naja) venom exhibits a serotonin-sensitive aryl acylamidase activity. Both acetylcholinesterase and aryl acylamidase activities co-eluted in column chromatographic procedures (Sephadex G-75 and Zinc-Sepharose), co-migrated on polyacrylamide gel electrophoresis, co-immunoprecipitated by anti-snake venom antibody and showed the same heat denaturation profile at 40 degrees C. Further, several potent acetylcholinesterase inhibitors at different
more » ... t different concentrations inhibited the cholinesterase and aryl acylamidase activities to the same extent. It is concluded that in cobra venom, acetylcholinesterase is associated with a serotonin-sensitive aryl acylamidase activity similar to earlier observations made with acetylcholinesterase from different sources.
pmid:22900296 fatcat:hrm5xlbgfzfqpaexsyjtci3dpi