Ion mobility-high resolution mass spectrometry in doping control analysis. Part II: Comparison of acquisition modes with and without ion mobility

Kateřina Plachká, Julian Pezzatti, Alessandro Musenga, Raul Nicoli, Tiia Kuuranne, Serge Rudaz, Lucie Nováková, Davy Guillarme
2021 Analytica Chimica Acta  
Analytica Chimica Acta 1175 (2021) 338739 performance level (MRPL) criteria of the World Anti-Doping Agency (WADA) when the DIA-IMS mode was applied. Automated data processing is important in routine doping analysis. Therefore, processing methods were optimized and evaluated for the prevalence of false peak assignments by analysing the target substances at different concentrations in urine samples. Overall, a significantly higher number of misidentified compounds was observed in Group II, with
more » ... in Group II, with an almost 2-fold higher number of misidentifications in DIA compared to DIA-IMS. This result highlights the benefit of the IMS dimension to reduce the rate of false positive in screening analysis. The optimized UHPLC-IM-HRMS method was finally applied to the analysis of urine samples from administration studies including nine doping agents from both Groups. However, to limit the number of interferences from the biological matrix, an emphasis is needed on the adequate settings of the data processing method.
doi:10.1016/j.aca.2021.338739 fatcat:orfzy52zwfbhpkgnicjerss464