Genetic Variants and Allele Frequencies of Kappa Casein in Egyptian Cattle and Buffalo Using PCR-RFLP

Eman M. Gouda, Mona Kh. Galal, Samy A. Abdelaziz
2013 Journal of Agricultural Science  
Kappa casein (K-Ca) genetic variations affected quality and composition of the milk. Several variants of Kappa casein (K-Ca) gene locus IV have been reported with special interest for the 'B' allele for its relation to the milk protein and fat yields. Genotyping and allelic frequencies of K-Ca among Native Egyptian breeds of cattle and buffalo were the aim of the present study. PCR amplification of DNA isolated from 300 blood samples collected from Holstein and Baladi cattle and buffalo were
more » ... formed followed by restriction fragment length polymorphism using Hind-III restriction endonuclease (PCR-RFLP). Detection of 'AA' and 'AB' genotypes in cattle breeds, 'BB' and 'AB' in buffalo and two alleles 'A' and 'B' in the studied breeds. Molecular selection for animals carrying the 'B' allele could impact breeding programs for dairy production in native cattle and buffalo breeds in Egypt.
doi:10.5539/jas.v5n2p197 fatcat:qh47nnifwnb5xfyvqgaypg66rq