Advances and challenges in genetic technologies to produce single-sex litters

Charlotte Douglas, James M. A. Turner, Jacob L. Mueller
2020 PLoS Genetics  
There is currently a requirement for single-sex litters for many applications, including agriculture, pest control, and reducing animal culling in line with the 3Rs principles: Reduction, Replacement, and Refinement. The advent of CRISPR/Cas9 genome editing presents a new opportunity with which to potentially generate all-female or all-male litters. We review some of the historical nongenetic strategies employed to generate single-sex litters and investigate how genetic and genome editing
more » ... ques are currently being used to produce all-male or all-female progeny. Lastly, we speculate on future technologies for generating single-sex litters and the possible associated challenges.
doi:10.1371/journal.pgen.1008898 pmid:32701961 fatcat:igdlbfkkj5hhjll7cuuuyw7a7u