Global gene expression analysis using Affymetrix GeneChip Human Transcriptome Array (HTA) 2.0, combined with Affymetrix Transcriptome Analysis Console (TAC) software, empowers scientists not only to gain information about RNA expression at the genelevel, but also about RNA alternative splicing at the exon-level in different biological samples. Real-time PCR is routinely used to verify microarray results due to its high sensitivity and wide dynamic range. Our R&D team validated GeneChip HTA 2.0

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... ene-level fold change and alternative splicing data by using the USB First-Strand cDNA Synthesis Kit [PN 75780] followed by real-time PCR with USB VeriQuest Probe or SYBR ® Green qPCR master mixes [PN 75650/75600]. We provide guidelines for this validation and show excellent inter-platform concordance between the microarray and the real-time PCR data.