A simple, rapid and sensitive spectrophotometic method for the estimation of trace amounts of salbutamol sulphate (SBS) in pharmaceutical preparations has been proposed .The method is based on the coupling reaction of the intended compound with diazotized 5-amino-2chlorobenzotrifluoride in alkaline medium and in the presence of Triton X-100 to form a yelloworange dye that shows maximum absorption at 467 nm. Beer's law is obeyed over the range 5 -300 µg / 10 ml (i.e.,0.5-30 ppm) with a molar

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... rptivity of 4.20 × 10 4 l.mol -1 .cm -1 and Sandell's sensitivity index of 0.0139 µg.cm -2 , a relative error of +1.08 to +3.46 % and a relative standard deviation of ±0.76 to ±3.50 %, depending on the concentration. The method has been applied to estimate salbutamol sulphate in syrup, tablet and ventolin. Keywords: Salbutamol sulphate, diazotized 5-amino-2-chlorobenzotrifluoride, diazo-coupling, Triton X-100, spectrophotometry ‫ــــــــــــــــــــــــــــــــــــــــــــ‬ ‫ــــــــــــــ‬ ‫ـــــــ‬ Al-Abachi, M.Q.; Al-Ghabsha, T.S. (1986). "Fundamentals of Analytical Chemistry". Mosul University Press, p.197 (in Arabic). Al-Abachi, M.Q.; Subhi, S. (2013). Flow injection-spectrophotometric determination of salbutamol sulphate and pyridoxine hydrochloride using 2,4-dinitrophenylhydrazine. Iraqi J. Sci., 54(1), 6-16. Al-Hafith, H.A. (2005). Development of spectrophotometric methods for the determination of some phenolic compounds and catecholamines in pharmaceutical preparations. M.Sc. Thesis, Mosul University, 45 p. Attaran, M.A.; Javanbakht, M.; Fathollahi, F.; Enhessari, M. (2012). Determination of salbutamol in pharmaceutical and serum samples by adsorptive stripping voltammetry on a carbon paste electrode modified by iron titanatenano powders. Electroanal., 24 (10), 2013-2020. Bao, Y.; Yang, F.; Yang, X. (2012). Capillary electrophoresis coupled with electrochemiluminescence for the facile separation and determination of salbutamol and clenbuterol in urine. Electroanal., 24(7),1597-1603. Basavaiah, K.; Prameela, H.C. (2003). Spectrophotometric determination of salbutamol sulfate and pyrantel pamoate in bulk drugs and pharmaceuticals. ; Stepnowski, P.; Kwiatkowski, M.; Migowska, N. (2011). Determination of b-blockers and b-agonists using gas chromatography and gas chromatography-mass spectrometry -A comparative study of the derivatization step. J. Chromatogr. A. 1218(44), 8110-8122. Dave, H.N.; Mashru, R.C.; Patel, A.K. (2011). Thin Layer chromatography method for the determination of ternary mixture containing salbutamol sulphate, bromhexine hydrochloride and etofylline. Edn., Singapore, p.498. Eswarudu, M.M.; Sushma, M.; Sushmitha, M.; Yamini, K. (2012). Validated spectrophotmetric method for the determination of salbutamol sulphate in bulk and pharmaceutical dosage forms. Int. Res. J. Pharma., 3(4), 423-425. Fattah, E.; Grant, D.; Gabr, K. ; Meshali, M. (1998). Physical characteristics and release behaviour of salbutamol sulphate beads prepared with different ionic poly-saccharides. Drug Devel. Indust. Pharma., 24(6), 541 -547. Ganjali, M.R.; Norouzi, P.; Ghorbani, M.; Sepehri C.R. (2005). A fourier transform cyclic voltammetric technique for monitoring ultratrace amounts of salbutamol at gold ultra microelectrode in flowing solutions Talanta, 66(5), 1225-1233. Ghulam, M.; Mahmood, A.; Muhammad, A.; Muhammad, W. (2009). A new reverse phase HPLC method with fluorescent detection for the determination of salbutamol sulphate in human plasma. Bull. Chem. Soc. Ethiopia, 23(1), 1-6. Kanakapura, I.; Huiikal, C. (2003). Spectrophotometric determination of salbutamol sulphate and acyclovir using iron(III) and ferricyanide., Sci. Asia, 29,141-146. Li, C.; Wu, Y.L.; Yang, T.; Zhang, Y. (2010). Simultaneous determination of clenbuterol, salbutamol and ractopamine in milk by reversed-phase liquid chromatography tandem mass spectrometry with isotope dilution. J. Chromatogr. A , 1217(50), 7873-7877 .