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Human endothelial cells from umbilical cord vein were grown to confluence on glass coverslips placed in Petri di= shes, in the presence or absence of dexamethasone. After ten days in culture, the cell monolayers were washed and treated with 0,5% Triton X lOO in Hank's solution , The detergent-treated slides were then washed in Hank's and examined by indirect immunofluorescence for fibronectin utilizing monospecific antibodies. Basement membranes produced by dexamethasone-treated cells showed adoi:10.1055/s-0038-1652348 fatcat:ma2waenj5fbepnmxpqvjmu6kka