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Enumerating algal viruses by flow cytometry v2
[dataset]
2016
protocols.io
1 1 1 VERVE Net Worden Lab Purpose : Purpose : To quantify virus-like particles in culture or environmental samples. Summa ry: Summa ry: Host cells and virus-like particles are enumerated from the same glutaraldehyde-fixed sample. For virus counts, an aliquot is diluted in 0.02-µm-filtered TE buffer prior to staining with SYBR Green I nucleic acid dye to improve staining efficiency and ensure an appropriately low event rate in the instrument. An unstained aliquot of sample is run for host cell
doi:10.17504/protocols.io.guibwue
fatcat:6qj2dtejrnfmrhdwyk2qxczwaq