INTERNATIONAL JOURNAL OF CHEMICAL, MATERIAL AND ENVIRONMENTAL RESEARCH Effect of Oxalic Acid on Cr 3+ Ion Uptake, Accumulation and Oxidative Stress by Sorrel (Hibiscus Sabdariffa L.) Seedlings in Hydroponic Solution

J Ogunleye, M Dagari, O Balogun, A Maigari
International Journal of Chemical, Material and Environmental Research   unpublished
The aim of this study is to investigate the effects of oxalic acid on the uptake, accumulation and oxidative stress of Cr 3+ ion by sorrel (Hibiscus sabdariffa L.) seedlings in hydroponic solution. The growth of 8 weeks old seedlings exposed to 0, 5, 10, 20 and 40 mg/L Cr 3+ ion and 20.0 mM oxalic acid was monitored in a greenhouse under controlled conditions. The application of oxalic acid for 10 days prior to harvest revealed that Cr 3+ ion accumulated significantly in the roots (p < 0.05)
more » ... h only a little translocated to the shoots. Hibiscus sabdariffa L. accumulated high Cr 3+ ion concentration (344.44 mg/kg) in the root at a concentration of 40 mg/L.The application of oxalic acid had inhibitory effects on the root and shoot biomass compared with that in the control. Therefore, chelation enhanced Cr 3+ ion uptake by Hibiscus sabdariffa L. The Cr 3+ ion-induced proline accumulation in shoots was also determined. There is no much difference in proline content of both the chelated and un-chelated treatment, however, the proline content decreased slightly with increasing Cr 3+ ion in the nutrients solution. Free proline is known to accumulate in plants under heavy metals exposure and is considered to be involved in stress resistance.