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Cholera Toxin Subunit B for Sensitive and Rapid Determination of Exosomes by Gel Filtration
2020
Membranes
We developed a sensitive fluorescence-based assay for determination of exosome concentration. In our assay, Cholera toxin subunit B (CTB) conjugated to a fluorescence probe and a gel filtration technique (size-exclusion chromatography) are used. Exosomal membranes are particularly enriched in raft-forming lipids (cholesterol, sphingolipids, and saturated phospholipids) and in GM1 ganglioside. CTB binds specifically and with high affinity to exosomal GM1 ganglioside residing in rafts only, and
doi:10.3390/membranes10080172
pmid:32751790
fatcat:4v5ev5vjqfaulgyoqijj4jwvge