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Expression and genomic integration of transgenes after Agrobacterium-mediated transformation of mature barley embryos
Genetics and Molecular Research
Genomic integration of transgenes in mature barley embryos expressed neomycin phosphotransferase II gene following AGL1 transformation. Ten randomly selected T 0 transformants were analyzed using thermal asymmetric interlaced polymerase chain reaction and 24 fragments ranged between 200-600 base pairs were sequenced. Three of the sequences flanked with transferred-DNA showed high similarity to coding regions of the barley genome, including alpha tubulin5, homeobox 1, and mitochondrial 16Sdoi:10.4238/2015.february.6.13 pmid:25730049 fatcat:hn3bv4sav5cmdfszods2zibl2a