Expression and genomic integration of transgenes after Agrobacterium-mediated transformation of mature barley embryos

C. Uçarlı, F. Tufan, F. Gurel
2015 Genetics and Molecular Research  
Genomic integration of transgenes in mature barley embryos expressed neomycin phosphotransferase II gene following AGL1 transformation. Ten randomly selected T 0 transformants were analyzed using thermal asymmetric interlaced polymerase chain reaction and 24 fragments ranged between 200-600 base pairs were sequenced. Three of the sequences flanked with transferred-DNA showed high similarity to coding regions of the barley genome, including alpha tubulin5, homeobox 1, and mitochondrial 16S
more » ... We observed 70-200-base pair filler sequences only in the coding regions of barley in this study.
doi:10.4238/2015.february.6.13 pmid:25730049 fatcat:hn3bv4sav5cmdfszods2zibl2a