Efecto antibacteriano de la asociación de hidróxido de cálcio y iodoformo sobre Enterococcus faecalis y Pseudomonas aeruginosa

Daniel Rodrigo Herrera Morante, Lidia Yileng Tay Chu Jon, Carlos Kose-Jr, Tami Momose Corrêa de Andrade, Eluise Cristina de Rezende, Vitoldo Antonio Kozlowski-Jr, Elizabete Brasil dos Santos
2014 Revista Estomatológica Herediana  
Antibacterial effect of the association of calcium hydroxide with iodoform on Enterococcus faecalis and Pseudomonas aeruginosa ABSTRACT The aim of this study was to assess whether there is antibacterial synergism on Enterococcus faecalis and Pseudomonas aeruginosa when iodoform was mixed to calcium hydroxide. A bacterial suspension containing 108 cells.ml of Enterococcus faecalis (ATCC 1495) and Pseudomonas aeruginosa (ATCC 9027), were plated on Müller Hinton agar, in duplicate, with a sterile
more » ... wab. After 10 minutes at 37°C, 4 holes of 5 mm in diameter were prepared in the agar and filled with CaOH2, iodoform and the association of both, mixed with 4 different vehicles: physiologic solution, 2% lidocaine with epinephrine, polyethylene glycol 400 and paramonochloro-phenol. The plates were incubated at 37°C during 48 hours and then the halos demonstrating inhibition of bacterial growth were measured. Using Tukey's test, the results showed that iodoform had antibacterial effect only when used as a vehicle of paramonochlorophenol, being this action attributed to the vehicle, which was similar to the antibacterial effect shown by calcium hydroxide pure and mixed with iodoform (p> 0.05). Iodoform showed greater inhibition against P. aeruginosa compared to E. faecalis. There was no statistically significant difference between the antibacterial effect of CaOH2 pure and mixed with iodoform, regardless the vehicle used. We conclude that the antibacterial effect shown by CaOH2 on E. faecalis and P. aeruginosa prevails over that of iodoform and their association does not alter this result.
doi:10.20453/reh.v18i1.1848 fatcat:gltz5wbohrhhnmkeeswfwveesu