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The development of a sustainable product using natural resources will gain much attention nowadays. This work aimed to study the effect of aqueous enzymatic extraction (AEE) using a different ratio of enzyme cocktails HEL1 and X7 towards the production of Momordica charantia L. (M. charantia) seed oil and to identify the composition of the seeds' lignocellulosic biomass (extracted-free materials, EFM). The M. charantia seed oil contained omega-3 fatty acids, such as stearidonic acid, thatdoi:10.48048/wjst.2021.9098 fatcat:slfevtfgcre7visap6yh3m4jry