A case of subcutaneous phaeohyphomycosis caused by Exophiala equina is reported in a 75year-old female, who showed subcutaneous abscesses on both forearms for 8 months. A lesion was initiated by inoculation with a spine from a tree. Histopathologically, suppurative granulomatous inflammation was present and short hyphal elements were observed. Upon culture greyish-black, velvety colonies of a black yeast were obtained after 3 weeks. The strain grew well at 25 6C, but poorly at 37 6C. After

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... ncing the internal transcribed spacer domain and the partial b-tubulin gene, the fungus was identified as E. equina. The patient was successfully treated with fluconazole for 3 months. The GenBank/EMBL/DDBJ accession numbers for the ITS and BT2 sequences of the E. equina isolate are JQ797584 and JQ797585, respectively.