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A novel biomolecular detection method, charge pumping, is investigated comprehensively as a biosensing mechanism. Tunneling electrons located in channel interface are responsible for a charge pumping current ͑I cp ͒ in a biosensing operation. The maximum tunneling distance ͑d tunnel ͒ of the electrons is the dominant factor to determine the amount of generated I cp , which is closely correlated with the sensitivity in a proposed nanogap-embedded biotransistor structure. Using an analyticaldoi:10.1063/1.3443580 fatcat:elno5yncwfavnkqrhrv5hid4wm