We present a comprehensive laser tweezers software package, comprising the software components for any laser tweezers system. This includes fast hologram generation software, implemented on a graphics card, thereby allowing 100 hundred independently moving traps at video frame rate. The software also includes comprehensive camera suite and image recognition software for multi-particle tracking and analysis. The software is freely available from the authors and

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... /Optics/). Downloaded From: http://proceedings.spiedigitallibrary.org/ on 11/30/2015 Terms of Use: http://spiedigitallibrary.org/ss/TermsOfUse.aspx Proc. of SPIE Vol. 7400 74001N-3 Downloaded From: http://proceedings.spiedigitallibrary.org/ on 11/30/2015 Terms of Use: http://spiedigitallibrary.org/ss/TermsOfUse.aspx Proc. of SPIE Vol. 7400 74001N-4 Downloaded From: http://proceedings.spiedigitallibrary.org/ on 11/30/2015 Terms of Use: http://spiedigitallibrary.org/ss/TermsOfUse.aspx Proc. of SPIE Vol. 7400 74001N-5 Downloaded From: http://proceedings.spiedigitallibrary.org/ on 11/30/2015 Terms of Use: http://spiedigitallibrary.org/ss/TermsOfUse.aspx