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Automated imaging of extended tissue volumes using confocal microscopy
2005
Microscopy research and technique (Print)
Confocal microscopy enables constitutive elements of cells and tissues to be viewed at high resolution and reconstructed in three dimensions, but is constrained by the limited extent of the volumes that can be imaged. We have developed an automated technique that enables serial confocal images to be acquired over large tissue areas and volumes. The computer-controlled system, which integrates a confocal microscope and an ultramill using a high-precision translation stage, inherently preserves
doi:10.1002/jemt.20200
pmid:16170824
fatcat:iyhz3thagfcbncx7ubssz7gm6y