(Z)-Ethylidene-4, 6-Dimethoxycoumaran-3-One Induces Apoptosis in Chronic Myelogenous Leukemia Cell Line

Joseph Madassery, Sanith Cheriyamun, Rahul Raghavan, K.B. Megha
2015 International Journal of Biological Chemistry  
The present study was performed to evaluate the antiproliferative property of (Z)-ethylidene-4, 6-dimethoxycoumaran-3-one (EDC), isolated from Pogostemon quadrifolius (Benth.) leaf against chronic myelogenous leukemia cell line (K-562). The purity and identity of the compound were confirmed by HPLC and ESI-MS analysis. Cell viability assay (MTT) was performed to evaluate the antiproliferative effect of EDC on K-562 cell line. The result showed that EDC inhibited the proliferation of K-562 cell
more » ... tion of K-562 cell line with an IC 50 of 7.77±0.21 µg mLG 1 (35.3 µM). Further DNA fragmentation assay was performed to identify the mode of action of EDC against K-562 cell line. The clear laddering pattern obtained after treating the cell line for 24 h with 50 µM EDC indicated that EDC induces cell death in K-562 cells by inducing apoptosis. Thus, the present study evidenced the potential antiproliferative property of EDC against chronic myelogenous leukemia cancer in vitro.
doi:10.3923/ijbc.2015.86.91 fatcat:eh6frrm7j5gdtotot5diwccyvy