A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2020; you can also visit <a rel="external noopener" href="http://doc.rero.ch/record/325377/files/10858_2014_Article_9841.pdf">the original URL</a>. The file type is <code>application/pdf</code>.
<i title="Springer Nature">
<a target="_blank" rel="noopener" href="https://fatcat.wiki/container/cimmcbrwkbf4xblwfxyaslsraa" style="color: black;">Journal of Biomolecular NMR</a>
A procedure is presented for automated sequence-specific assignment of NMR resonances of uniformly [ 13 C, 15 N]-labeled RNA. The method is based on a suite of four through-bond and two through-space highdimensional automated projection spectroscopy (APSY) experiments. The approach is exemplified with a 0.3 mM sample of an RNA stem-loop with 48 nucleotides, K10, which is responsible for dynein-mediated localization of Drosophila fs(1)K10 mRNA transcripts. The automated analysis of the APSY data<span class="external-identifiers"> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1007/s10858-014-9841-3">doi:10.1007/s10858-014-9841-3</a> <a target="_blank" rel="external noopener" href="https://www.ncbi.nlm.nih.gov/pubmed/24899400">pmid:24899400</a> <a target="_blank" rel="external noopener" href="https://fatcat.wiki/release/xctcz7dlcvgbracadgkra3wxne">fatcat:xctcz7dlcvgbracadgkra3wxne</a> </span>
more »... led to highly accurate and precise 3-to 4-dimensional peak lists. They provided a reliable basis for the subsequent sequence-specific resonance assignment with the algorithm FLYA and resulted in the fully automated resonance assignment of more than 80 % of the resonances of the 13 C-1 H moieties at the 1 0 , 2 0 , 5, 6, and 8 positions in the nucleotides. The procedure was robust with respect to numerous impurity peaks, low concentration of this for NMR comparably large RNA, and structural features such as a loop, single-nucleotide bulges and a non-Watson-Crick wobble base pairs. Currently, there is no precise chemical shift statistics (as used by FLYA) for RNA regions which deviate from the regular A-form helical structure. Reliable and precise peak lists are thus required for automated sequence-specific assignment, as provided by APSY.
<a target="_blank" rel="noopener" href="https://web.archive.org/web/20200307153858/http://doc.rero.ch/record/325377/files/10858_2014_Article_9841.pdf" title="fulltext PDF download" data-goatcounter-click="serp-fulltext" data-goatcounter-title="serp-fulltext"> <button class="ui simple right pointing dropdown compact black labeled icon button serp-button"> <i class="icon ia-icon"></i> Web Archive [PDF] <div class="menu fulltext-thumbnail"> <img src="https://blobs.fatcat.wiki/thumbnail/pdf/a6/f9/a6f92f808c0868f81aa8e551e5f142380b31e28d.180px.jpg" alt="fulltext thumbnail" loading="lazy"> </div> </button> </a> <a target="_blank" rel="external noopener noreferrer" href="https://doi.org/10.1007/s10858-014-9841-3"> <button class="ui left aligned compact blue labeled icon button serp-button"> <i class="external alternate icon"></i> springer.com </button> </a>